Catalog Number - 40031-25P

40031-25P Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), providing a uniquely powerful and versatile mixed‑mode separation mechanism that goes far beyond traditional reversed phase or ion exchange columns.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling three primary interaction modes:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This combination provides exceptional selectivity for basic, polar, and ionizable compounds, making it highly effective for complex matrices and difficult separations where conventional phases fail.

The TYPE‑C silica hydride base offers significant performance advantages:

• Direct silicon–carbon bonding for superior stability

• Operates at very low pH (<2) without degradation

• Faster equilibration compared to traditional silica columns

• Reduced sensitivity to mobile phase moisture (beneficial for HILIC methods)

250 mm Length – Maximum Resolution for Complex Analytical Challenges

The 250 mm length delivers the highest resolving power in this column family, ideal for demanding separations.

• Maximum resolution compared to shorter column formats

• Superior separation of closely related or co‑eluting compounds

• Increased retention and peak capacity for complex samples

✅ Recommended for impurity profiling, method development, and separations requiring extended retention and maximum resolution

Flow Rate & System Requirements (4.6 mm ID, 4 µm)

✅ Fully compatible with standard HPLC systems

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Moderate backpressure relative to column length

• Compatible with UV and MS detectors

• Suitable for routine and advanced analytical workflows

✅ Best Practice: Use proper sample filtration and a guard column to optimize performance and extend column life

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting compounds and structural isomers

• Effective for basic and positively charged analytes

pH‑Driven Selectivity Control

• WCX functionality active at pH ≥ 6

• Retention can be tuned via pH and ionic strength

✅ Ideal when C18, phenyl, or traditional ion exchange columns cannot achieve sufficient separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under harsh conditions including very low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces analysis cycle times

No Strict Moisture Control (HILIC)

• Simplifies mobile phase preparation

Extended Column Lifetimes

• Durable for long‑term use

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in a single column

Standard HPLC Compatibility (4 µm)

• No need for UHPLC systems

• Lower pressure vs sub‑2 µm columns

Maximum Resolution Capability

• Ideal for highly complex and demanding separations

Typical Applications

• Complex matrix separations

• Impurity profiling and method development

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Compatible with standard HPLC systems

• Suitable for both hydrophilic and hydrophobic analytes

• pH control is critical for WCX selectivity optimization